Evaluation of high dilutions extract of pyroligneous on the development of Colletotrichum gloeosporioides

Abstract

The aim of this research was to evaluate the fungitoxicity of high dilutions of tectonic (Tectona grandis) extract in mycelial growth, sporulation and germination of the fungus Colletotrichum gloeosporioides. In a completely randomized design with six dynamizations of the pyroligneous extract (3, 9, 15, 21, 27 and 33 CH) and three controls (distilled water, mother tincture, 30% hydroalcoholic solution) with four replicates each. All treatments were diluted to 0.1% in BDA medium. Mycelial growth rate index, relative percentage of mycelial development (PRD%), inhibition of sporulation and spore germination (%) were evaluated. The treatments did not differ among them for mycelial growth, percentage of spore germination and IVCM. The PRD and sporulation variables varied according to the dynamization, with a maximum reduction of 7% in PRD (33 CH) and a 70% increase in sporulation (3 and 21 CH). The dynamized pirolenous extract did not present expressive fungitoxicity in the growth, sporulation and germination of C. gloeosporioides.