Fungitoxicity of the Pyroligneous Extract in the Development in vitro of Colletotrichum gloeosporioides

Cleverson Rodrigues,João Aguilar Massaroto,Marco Antonio Camillo De Carvalho,Walmor Moya Peres,Dilânia Lopes De Matos,Grace Queiroz David,Oscar Mitsuo Yamashita

doi:10.5296/jas.v8i4.17009

Cleverson Rodrigues, João Aguilar Massaroto + Show 5 more

Open Access

https://doi.org/10.5296/jas.v8i4.17009

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Abstract

Products derived from renewable sources have been studied for their use in agriculture enabling increments in the production of agricultural culture, such as in the case of the pyroligneous extract. The research aimed at evaluating the fungitoxic potential of the pyroligneous extract of teak (Tectona grandis L.) on mycelial growth, sporulation and spore germination of Colletotrichum gloeosporioides. The experiment was conducted in completely randomized design with five treatments and four replications. Five doses of pyroligneous extract were tested (0, 25, 50, 100, 150 mL L-1) following the pour-plate methodology in 10 mL of potato dextrose agar media and kept in the incubating room maintained at 25 ± 2 °C in a 12 hour-photoperiod for eight days. Mycelial growth variables (cm), mycelial index growth speed, growth inhibition (%), sporulation inhibition (%) and spore germination (%) were evaluated. There was a reduction for the mycelial growth as well as for the rate of mycelial growth, sporulation inhibition and spore germination as the doses were increased. The percentage inhibition of mycelial growth was 56 % for the highest dose (150 mL L-1). Pyroligneous extract of teak has a direct fungitoxic action on growth, sporulation and germination in vitro of the fungus C. gloeosporioides.

Highlights

In modern agriculture, the viability of using products from renewable sources has been studied, enabling increases in the production of agricultural crops (Grewal et al, 2018).One of these products is pyroligneous extract, obtained by condensation of the smoke released in the process of carbonization of wood for charcoal production
The research aimed at evaluating the fungitoxic potential of the pyroligneous extract of teak (Tectona grandis L.) on mycelial growth, sporulation and spore germination of Colletotrichum gloeosporioides
The research comprised two stages, the first to verify the in vitro fungitoxic action of pyroligneous extract doses on mycelial growth and sporulation of the fungus Colletotrichum gloeosporioides and the second to verify these doses on spore germination

Summary

Introduction

The viability of using products from renewable sources has been studied, enabling increases in the production of agricultural crops (Grewal et al, 2018).One of these products is pyroligneous extract, obtained by condensation of the smoke released in the process of carbonization of wood for charcoal production. The research aimed at evaluating the fungitoxic potential of the pyroligneous extract of teak (Tectona grandis L.) on mycelial growth, sporulation and spore germination of Colletotrichum gloeosporioides.

Results

Conclusion