Abstract
Aim- The aim of this in-vitro study was to compare the effect of NaOCl, EDTA, Propolis and Distilled water on cyclic fatigue resistance of Protaper Gold rotary NiTi instruments.
 Method- A total of 40 commercially available rotary (n =40) Ni-Ti files were used to assess the effect of different root canal irrigants on their cyclic fatigue resistance. The files were divided into four groups (n=10 per group) as Group 1- Distilled water (Control group), Group 2 – 5.25 % NaOCl, Group 3 -17% EDTA and Group 4 - Propolis. The instruments were rotated in custom-made stainless steel artificial canal (angle of 60˚and 5 mm radius), fixed in a glass container with irrigating solution until the instrument fractured. The time to fracture was registered for each instrument and the number of cycles to fracture (NCF) was calculated and statistical analysis was done.
 Result- NiTi files used with Group 1 (Distilled water) exhibited best cyclic fatigue resistance (1386.50±37.12) amongst all the tested groups followed by Group 4 (Propolis) and Group 3 (EDTA). Group 2 (5.25 % NaOCl) showed least cyclic fatigue resistance (971.50 ± 48.879) compared to other groups (P>0.05). 
 Conclusion- NiTi files exhibited best cyclic fatigue resistance when used with distilled water and NaOCl irrigating solution significantly deteriorated the surface characteristics and cyclic fatigue resistance of rotary NiTi files.
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