Abstract
ABSTRACTCluster BE1 Streptomyces bacteriophages belong to the Siphoviridae, with genome sizes over 130 kbp, and they contain direct terminal repeats of approximately 11 kbp. Eight newly isolated closely related cluster BE1 phages contain 43 to 48 tRNAs, one transfer-messenger RNA (tmRNA), and 216 to 236 predicted open reading frames (ORFs), but few of their genes are shared with other phages, including those infecting Streptomyces species.
Highlights
Cluster BE1 Streptomyces bacteriophages belong to the Siphoviridae, with genome sizes over 130 kbp, and they contain direct terminal repeats of approximately 11 kbp
KM652554 MF155946 MF347636 MF347637 MF347638 MF347639 MF358542 MF358541 tions were assigned using BLASTP [4] and HHpred [5] or by protein-threading assessments using Phyre2 [6]; potential functions were assigned to approximately 20% of the protein-coding genes
The direct terminal repeats of approximately 11 kbp are the longest found in any of the actinobacteriophages to date, and they are similar in size to previously reported long repeats in phages, such as T5 and SPO1, which have terminal repeats of 10,139 and 13,185 bp, respectively [7, 8]
Summary
Cluster BE1 Streptomyces bacteriophages belong to the Siphoviridae, with genome sizes over 130 kbp, and they contain direct terminal repeats of approximately 11 kbp. All genomes were annotated using Glimmer and GeneMarkS and refined using BLAST alignments to previous manually annotated genes; start codon choice was evaluated using multiple sequence alignments of closely related genes. L.E.H., C.D.S., and V.C.W. contributed to this article.
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