Effect of long noncoding RNA PANDAR on the proliferation and invasion of breast cancer cells

Abstract

Objective To investigate the effect of long noncoding RNA (lncRNA) PANDAR on the proliferation and invasion of breast cancer cells. Methods We constructed the stable MCF-7 cells with overexpression or knockdown of PANDAR by using lentivirus system and detected the cell proliferation and invasion by cell counting kit-8 (CCK-8) assay and matrigel Transwell assay. Results The relative expression of PANDAR in MCF-7 cells with overexpression of PANDAR was 20.05±1.08, higher than in the control group (P=0.000), and that in MCF-7 cells with knockdown of PANDAR was 0.16±0.02, lower than in the control group (P=0.008). The cell proliferation activity of PANDAR overexpression group (0.489±0.067, 0.945±0.025, 1.236±0.072 ) at 24, 48 and 72 h, were higher than these in control group (0.385±0.024, 0.576±0.059, 0.737±0.041; P=0.045, P=0.024, P=0.012, respectively). The cell proliferation activity of PANDAR knockdown group (0.292±0.055, 0.371±0.041, 0.455±0.068) at 24, 48 and 72 h, were significantly lower than these in control group (P=0.036, P=0.030, P=0.014), respectively. The amount of invasion cells in PANDAR overexpression group were significantly more than control group (48.37±13.08 vs. 25.15±6.75; P=0. 011). While the amount of invasion cells in PANDAR knockdown group were significantly less than control group (23.35±2.96 vs. 50.13±11.87; P=0. 015). Conclusion PANDAR promotes cell proliferation and invasion of breast caner cells. Key words: Long noncoding RNA; Breast cancer