WITHDRAWN: Long non-coding RNA LUCAT1 up-regulates the expression of HIF-1α and promotes the proliferation and metastasis of breast cancer cells via sponging miR-199a-5p

Abstract

Abstract Background Long non-coding RNA (lncRNA) lung cancer associated transcript 1 (LUCAT1) plays an important regulatory role in a variety of cancers, but its role in breast cancer is still unclear. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate the expression of LUCAT1, miR-199a-5p, HIF-1α in breast cancer tissues and cell lines; CCK8 method was used to detect the proliferation of breast cancer cells; Transwell assay was adopted to evaluate the migration and invasion of breast cancer cells; luciferase reporter gene assay was used to validate the interaction between LUCAT1 and miR-199a-5p. Western blot was utilized to analyze the effects of LUCAT1 and miR-199a-5p on the expression of HIF-1α. Results LUCAT1 was significantly up-regulated in breast cancer and cell lines; overexpression of LUCAT1 promoted the proliferation, migration and invasion of breast cancer cells. Additionally, LUCAT1 functioned as “ceRNA” to modulate the expression of miR-199a-5p, in turn regulated the expression of HIF-1α. Conclusion LUCAT1 is an oncogenic lncRNA in breast cancer, and it can inhibit the function of miR-199a-5p and increase the expression of HIF-1α, promoting the proliferation, migration and invasion of cancer cells.