Abstract
Objective To study the effects of salinomycin on the proliferation and invasion of breast cancer cells and the mechanism.Methods The human breast cancer cells MCF-7,MCF-7/ADR and MDA-MB-231,were treated with salinomycin.Cell viability was determined by cell counting kit-8.The invasive ability of tumor cells was evaluated by Transwell chamber assay.The mRNA and protein levels of ETS1 and matrix metalloproteinase (MMP)-1 were detected by using real-time quantitative polymerase chain reaction (Real-time PCR) and Western blotting respectively.Results Salinomycin significantly inhibited the proliferation of breast cancer cells with the IC50 value being (7.47 ±0.64) for MCF-7,(5.11 ±0.97) for MCF-7/ADR,and (4.08 ±0.85) μmol/L for MDA-MB-231,respectively.The invasion capacity of breast cancer cells were significantly reduced by salinomycin (65.9％/41.9％ for MCR-7,55.8％ /37.8％ for MCR-7/ADR,and 48.5％/24.6％ for MDA-MB-23I,respectivel).Simustaneously,the expression levels of ETS1 and MMP-1 were downregulated by salinomycin.Conclusion Salinomycin can inhibit the proliferation of breast cancer cells,and reduce the invasion capacity. Key words: Breast cancer; Salinomycin; Proliferation; Invasion
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
