Abstract
The aim of this research was to analyze the effect of kebar grass on the seminiferous tubules diameter and epithelium thickness of mice exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD). Twenty five BALB/C mice were used with five treatments (4 mice each group): Negative Control (C-), Positive Control (C+) with TCDD 7μg/kgBW IP, and treatment groups that treated with TCDD 7μg/kgBW IP and Kebar Grass orally for 53 days were presented T1 0,045mg/gBW/day, T2 0,080mg/gBW/day, and T3 0,135mg/gBW/day. Results showed that TCDD decreased the diameter and epithelium thickness of seminiferous tubules, while kebar grass extract was proven to maintain diameters but could not maintain epithelium thickness of of the seminiferous tubules Keywords : Kebar grass, Seminiferous tubule, Tetrachlorodibenzo-p-dioxin (TCDD), Mice
Highlights
2,3,7,8 - tetrachlorodibenzo-p-dioxin (TCDD) compounds have a wide enough toxic level that they can cause damage to the body tissues (Pelclová et al, 2006)
US Publishers widely known, the researchers wanted to observe the effect of kebar grass extract to the diameter and thickness of seminiferous tubule epithelium in mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin to observe potential kebar grass in counteracting the toxic effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin
The materials used in this study were male (Mus musculus) mice of BALB-C strain, kebar grass extract dose of 0.045 mg / gBB, 0.08 mg / gBB, and dose of 0.135 mg / gBB (Lefaan, 2014), solution of 2,3,7,8-tetrachlorodibenzo-p-dioxin (Supelco brand no. catalog 48599), corn oil, aquadest, 10% formalin buffer, 70% alcohol, xylol, water-soluble paint, and canada balsam
Summary
2,3,7,8 - tetrachlorodibenzo-p-dioxin (TCDD) compounds have a wide enough toxic level that they can cause damage to the body tissues (Pelclová et al, 2006). Research conducted by Lefaan (2014) showed that kebar grass contains three types of chemical compounds flavonoids, saponins, and tannins that have the potential to give effect to the process of spermatogenesis. It could increase the diameter and thickness of the seminiferous tubular epithelium (Lefaan, 2014) This improve that kebar grass is a potential material and good for male reproduction (Lefaan, 2014). US Publishers widely known, the researchers wanted to observe the effect of kebar grass extract to the diameter and thickness of seminiferous tubule epithelium in mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin to observe potential kebar grass in counteracting the toxic effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin
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