PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum) TERHADAP DIAMETER DAN TEBAL EPITEL TUBULUS SEMINIFERUS MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN

Abstract

The aim of this research is to analyze the effect of kebar grass on the diameter and epithelium thickness of seminiferous tubules. Twenty male mice of BALB/C strain with an average weight of 25 gram were used in this research. Total five experimental groups (4 mice each group) were treated with combination of TCDD and kebar grass extract designed as follows: (C-) 0mg/g BW/day dan 0µg/kg BW, (C+) 0mg/g BW/day and 7µg/kg BW, (T1) 0,045mg/g BW/day and 7µg/kg BW, (T2) 0,080mg/g BW/day and 7µg/kg BW, (T3) 0,1350mg/g BW/day and 7µg/kg BW. The TCDD was injected peritoneally in single dose while kebar grass extract was given orally everyday for 53 days. The results showed a significant difference (p<0,05) on group which is treated with kebar grass extract 0,135mg/ g BW/ day is proven could maintain the diameter of seminiferous tubules in T3 group (170,5μmb ± 0,65μm) compared with C+ group (129,50μma ± 8,42μm). Significant difference of epithelial thickness were also found between the control and treatment groups (p<0,05). The C+ group (33,57μmb ± 1,61μm) was different from the T1 group (43,80μmc ± 1,46μm), T2 (48,49μmd ± 1,08μm), T3 (52,15μme ± 0,86μm) and C- (56,17μma ± 1,35μm). This findings might suggest TCDD has the potential to decrease the epithelium thickness and the diameter of seminiferous tubules, while kebar grass extract in various dose were proven to be able maintain the epithelium thickness of seminiferous tubules but could not maintain the diameter of seminiferous tubules.