Kebar Grass Extract (Biophitum Petersianum) Increases Diameter Of The Seminiferous Tubules Of Diabetic Mice

Faraida Arvilla

doi:10.22219/sm.vol17.smumm1.12150

Abstract

Diabetes is a metabolic disease that can affect the male reproductive system. The condition of hyperglycemia will increase the production of ROS, causing oxidative stress. Oxidative stress causes microangiopathy, triggers cell apoptosis, interferes with spermatogenesis, thereby affecting the diameter of the seminiferous tubules. Kebar grass contains of flavonoid and vitamin E which can be used to reduce ROS reactions and inhibit oxidative stress. This research was to prove that kebar grass extract can increase diameter of the seminiferous tubules of diabetic mice. Total of 30 samples were divided into 5 groups: negative control group (K-) was given CMC-Na, positive control group (K+) was given metformin 2mg/kg, groups P1, P2 and P3 received 2mg/kg metformin and kebar grass extract with different dosages (67.5mg/kg, 135mg/kg and 270mg/kg). The treatment lasted for 35 days. On the 36th day the mice were sacrificed and the testes were taken, then histological observations were made with HE staining to measure the diameter of tubules. The result showed that there was a significant difference in the diameter of seminiferous tubules with p value=0,000. Kebar grass extract can increase the diameter of seminiferous tubules of diabetic mice.

Highlights

Diabetes mellitus is a global health problem because it affects more than 400 million people worldwide and is expected to increase to more than 600 million by 2045 (International Diabetes Federation, 2017)
The aim of this study is to prove the potential for administration of kebar grass extract (Biophytum petersianum Klotzsch) in increasing the diameter of the seminiferous tubules of diabetic mice
The aim of this study was to prove that administration of kebar grass extract can increase the diameter of the seminiferous tubules in diabetic mice

Summary

Introduction

Diabetes mellitus is a global health problem because it affects more than 400 million people worldwide and is expected to increase to more than 600 million by 2045 (International Diabetes Federation, 2017). Diabetes mellitus is a chronic metabolic disorder due to the pancreas not being able to produce enough insulin or the body cannot use insulin that has been produced effectively (Kementrian kesehatan, 2014). This disease is characterized byincreased glucose levels that exceed. Conditions of high glucose levels in the blood can cause excessive ROS formation in the body resulting in oxidative stress. Oxidative stress that occurs in the testes will result in damage to the mitochondrial membrane and cause loss of potential function of the mitochondrial membrane which will lead to cell death or apoptosis. That will interferes the process of spermatogenesis that occurs in the seminiferous tubules (La Vignera et al, 2015)

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Results