Efectivness of kebar grass extract (biophytum petersianum klotzch) in increasing thickness of the seminiferous tubular epithelium in male mice (mus musculus) diabetes mellitus model

Abstract

Background: Administration of antioxidants has been proven to reduce the production of free radicals in the body. One of the natural ingredients that contain antioxidants are Kebar grass. Kebar grass (Biophytum petersianum Klotzch) contains three types of chemical compounds that could potentially impact spermatogenesis. Those are flavonoids, saponins and tannins so it needs to investigate the effect on the testicles with a model of diabetes mellitus (DM). Purpose: Analyzed the Effectivness of Biophytum petersianum Klotzch extract to the epithelium thickness of the seminiferous tubules of mice (Mus musculus) model of diabetes mellitus. Methods: This research was a post test only control group design using Balb / C 45 male tail number, divided 5 groups were randomly selected. K-: DM mice group was given CMC Na 1% for 21 days. K +: DM mice group was given CMC Na 1% + metformin 2 mg / gBW for 35 days. (P1, P2, P3): DM group of mice given doses of 67.5 kebar seaweed extract, 135, 270 mg / kg + metformin 2 mg / gBW 35 days. Valid to 45 surgery and sampling and examination of thickness of seminiferous tubule epithelium. Results: The observation of thick epithelium of the seminiferous tubules was significant difference (p = 0.005) with a mean negative control group (K) 65 235 ± 5012, positive control(K +) 71 216 ± 12 514, (P1) 76 756 ± 6144, (P2) 80 660 ± 4140, (P3) 81 418 ± 7319. Conclusion Extract grass kebar (Biophytum petersianum Klotzch) efectivness in improving a thick epithelium of the seminiferous tubules in male mice model of diabetes mellitus. Keywords : Kebar grass; diabetes mellitus; thick epithelium of the seminiferous tubules.