Abstract
High amylose starch (HAMS) was employed as an effective carrier to incorporate thymol within pullulan nanofibers. Aqueous solutions with HAMS concentrations of 0% - 5% were investigated to study their effect on the incorporation of thymol during electrospinning. As a result, the loading capacity was significantly improved by the addition of HAMS, increasing from 2.38% to 6.15%. Thymol-starch mixture with an irregular structure was embedded within pullulan nanofiber according to SEM and TEM results. The HAMS-1, HAMS-3 and HAMS-5 were endowed with good anti-microbial inhibition against Escherichia coli and Bacillus cereus. Additionally, HAMS-5 exhibited sustained release of thymol, while the addition of HAMS improved the mechanical properties of HAMS-1. Further investigation into the interaction between HAMS, pullulan, and thymol in the nanofibers was conducted. HAMS proved effective in enhancing the bioavailability of thymol and provides a promising material for developing edible, sustainable polysaccharide antimicrobial nanofibrous films for the food industry.
Published Version
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