Abstract
Kluai Nam Thai’ (Musa AA group) are rare in Thailand and it is at risk of extinction because of environmental changes and human behavior. Hence, the plant tissue culture technique is used for propagation. This research aimed to study the effect of coconut water on root induction of Kluai Nam Thai in vitro. The shootlets from laboratory were cultured on Murashige and Skoog (MS) medium supplemented with coconut water at concentrations 10, 15, 20, and 25% for 1 month. The experimental design was Completely Randomized Design (CRD). The results indicated that MS medium supplemented with 20% coconut water gave the highest average number of roots at 1.85 while MS medium supplemented with 10% coconut water gave the highest length of roots at 2.34 cm which were significantly different (p<0.01) from the other concentrations
Highlights
In Thailand, banana grants have been grown since the ancient time
Growth regulators are important for the plant tissue culture
It was found that the coconut water with the concentration of 15% and indole-3-acetic acid (IAA) with the concentration of 0.25 ppm could result in the highest number of roots (10.66 roots)
Summary
In Thailand, banana grants have been grown since the ancient time. There are local and imported species. Auxin in the coconut water is an indole-3-acetic acid (IAA) that regulates the growths of plants as well as the developments of shoots, roots, raw coconuts and areas with many tissues [5], [6]. It was found that the MS medium supplemented with 15% coconut water could resulted in shoots induction for 100% and root average 1.40 roots per plant. According to the stated data, the plant tissue culture technique was used for reproducing „Kluai Nam Thai‟ by studying the effects of the coconut water on the root induction in vitro. The objective of this research aimed to study the effects of the coconut water on the root induction of „Kluai Nam Thai‟ in vitro
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