Abstract
Background: Pineapple fruit used in this study was obtained from Sipahutar sub-district, North Tapanuli regency, North Sumatra which was a superior commodity fruit. Propagation of the Sipahutar pineapple plant was conducted vegetatively using bud stems and crown shoots, but the amount was limited to be planted in large areas. Therefore, propagation through tissue culture techniques could be used as an alternative solution.Objective: Explants source were taken from plantlets in vitro aged 3 months were grown on Murashige and Skoog (MS) basic medium with the addition of Indole Acetic Acid (IAA) 1 ppm.Methods: Sipahutar pineapple tissue culture work has been done in YAHDI Plant Tissue Culture Laboratory, Medan. The study design used a factorial completely randomized design. The first factor is coconut water with concentration of 0%, 10%, 20%, and 30%, second factor is Benzyl Amino Purine (BAP) with concentration 0 ppm, 2 ppm and 4 ppm, so there were 12 combination with 3 repetition.Result: The results showed that the addition of coconut water on MS base medium with IAA of 1 ppm gave a significant effect on the number of leaf yield and shoot height. The provision of BAP on basic MS medium with IAA of 1 ppm gave a significant effect on the number of shoots produced, the number of leaves produced, the length of the leaves, the width ofleaves and the length of shoots. The interaction of coconut water and BAP addition on MS base medium with IAA of 1 ppm gave significant effect on leaf length, leaf width and shoot length as well.Conclusions: MS + IAA basic media combined with coconut water + BAP could stimulate the growth of Sipahutar pineapple in vitro. Â
Highlights
Pineapple (Ananas comosus (L.) Merr.) belongs to the Bromeliaceae family is a tropical plant originating from Brazil, Bolivia and Paraguay
The result of analysis of variance showed that the addition of Coconut water and Benzyl Amino Purine (BAP) had a significant effect on the number of leaves, while the interaction of Coconut water + BAP had no significant effect on the number of leaves
The result of analysis of variance showed that the addition of Coconut water and its interaction did not give a significant effect to the number of shoots, while the provision of BAP significantly affected the number of buds
Summary
Pineapple (Ananas comosus (L.) Merr.) belongs to the Bromeliaceae family is a tropical plant originating from Brazil, Bolivia and Paraguay. This fruit in English is called pineapple because its shape is like a pine tree. Tissue culture is one of the methods of in vitro plant propagation that utilizes the plant totipotential nature to obtain pineapple seeds in large quantities and in a short time (Yusnita, 2004; Harahap, 2011). The addition of Coconut water indicated to contain cytokines is expected to induce plant shoots (Harahap, 2011).The purpose of this study was to determine the effect of Coconut water and BAP addition to the growth of Sipahutar pineapple
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
