Effect of Addition Different Levels of Organic, Inorganic and Nano-Se to the Diet of Broiler Chickens (Ross 308) Exposed to Heat Stress on Characteristics Blood Cellular and some Indicators of Oxidative Stress

Abstract

This study was conducted at Al-Anwar Poultry Station in Babil Governorate Iraq for 35 days, from July 10, 2022 to August 17, 2022, in order to demonstrate effect of different concentrations of organic, inorganic, and nano-selenium added to the broiler diet on characteristics blood and under conditions of heat stress, 560 chicks of one day old (Ross 308) were used without identifying their sex and divided randomly into 7 treatments with 4 replicates for each treatment, and each replicate included 20 chicks. The first treatment, T1, was the control treatment without addition, and organic selenium was added in the second and third treatments (T2 & T3) concentration of (1 & 1.5 mg / kg), respectively, while the fourth and fifth treatment (T4 & T5) inorganic selenium was added concentration of (1 & 1. 5 mg / kg), respectively, while the sixth and seventh treatment (T6 & T7) included the addition of nano-Se concentration of (1 & 1.5 mg / kg), respectively. The results of study showed following: (1) T7 treatments outperformed all examined treatments in terms of Red blood cell count and Packed cell volume with highly significant (P≤0.01) and results of the statistical analysis didn’t show any significant difference between the studied treatments in the concentration of hemoglobin in the blood. (2) The results show that there was a high significant increase (p ≤ 0.01) in percentage of lymphocytes in all addition treatments compared to the control, unlike heterophil cells, and percentage of H/L whose percentages were significantly low in all addition treatments compared to the control treatment. (3) There was a significant increase (p ≤ 0.01) in the concentration of ALT and GSH enzyme in T2 compared to all treatments, while AST enzyme increased significantly (p ≤ 0.01) in the serum of T3 treated birds compared to control, also a significant increase in MDA observed in T4 over all studied treatments.