Abstract
Modification of a potato extract medium was undertaken to increase pollen embryogenesis from anthers of a hard red spring wheat (Triticum aestivum L. cv. Chris). Whole potato tubers (Solatium tuberosum L. cv. Kennebec) were separated into peeled tubers and peel from which extracts were prepared. A 3 ✕ 3 latin square design with three replications in time was used to compare media prepared from: (i) extract of whole potato tubers, (ii) extract of the peel only, and (iii) extract of peeled tubers. A 60% increase in anther induction resulted from the peeled tuber extract medium compared to the whole potato medium. Removal of the peel from tubers probably reduces the concentration of compounds that may be inhibitory to wheat embryogenesis during anther culture. Anther source, defined by spike section (basal, middle, and distal), was a second variable in the experiment but did not significantly affect embryogenesis.
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