Eco-Friendly Microwave Assisted Green Synthesis, Characterization and Antibacterial Activity of Silver Nanoparticles

Devendra Kumar,Neelam ,Akanksha Tiwari

doi:10.30799/jnst.246.19050402

Abstract

This paper deals a novel method for the synthesis of silver nanoparticles (AgNPs) by microwave assisted approach using silver nitrate and trisodium citrate. The formation of nanoparticles was confirmed by UV-visible spectral studies. Particle size and morphology was determined by P-XRD and HR-TEM studies which revealed the average particle size of synthesized nanoparticles 21.49 and 20.08 nm respectively with spherical shape. The synthesized nanoparticles were screened for antibacterial activity in vitro against gram-positive bacteria <i>Staphylococcus aureus</i> and <i>Bacillus subtilis </i>and gram-negative bacteria <i>Escherichia coli </i>and <i>Klebsiella pneumoniae</i> by adopting disk diffusion method. The results of antibacterial studies exhibited that AgNPs were potential antibacterial agent.<div><br></div>

Highlights

The biological activity of silver nanoparticles depends on numerous factors including size, shape dependent distribution, composition of particles, particles reactivity in solution, morphology, effectiveness of ion liberates, type of cell and the nature of reducing agents used for the synthesis of AgNPs [10]
The prepared silver nanoparticles have been screened for their antibacterial activity in vitro against gram +ve bacteria Staphylococcus aureus and Bacillus subtilis and gram –ve bacteria Escherichia coli and Klebsiella pneumoniae by adopting disk diffusion method
In the present paper ecofriendly microwave assisted synthesis method has been used for the synthesis of silver nanoparticles

Summary

Introduction

Metallic nanoparticles are unique and can significantly modify physical, chemical and biological properties compared to bulk material of same composition because of their surfaceto-volume ratio. These nanoparticles have been utilized for a variety of applications [2,3,4,5]. The biological activity of silver nanoparticles depends on numerous factors including size, shape dependent distribution, composition of particles, particles reactivity in solution, morphology, effectiveness of ion liberates, type of cell and the nature of reducing agents used for the synthesis of AgNPs [10]. The prepared silver nanoparticles have been screened for their antibacterial activity in vitro against gram +ve bacteria Staphylococcus aureus and Bacillus subtilis and gram –ve bacteria Escherichia coli and Klebsiella pneumoniae by adopting disk diffusion method

Objectives

Results

Conclusion