Abstract
ABSTRACTWe report here the draft whole-genome sequence of a fluorene-degrading bacterium, Sphingobium sp. strain LB126. The genes involved in the upper biodegradation pathway of fluorene are located on a plasmid, and the lower pathway that generates tricarboxylic acid cycle intermediates is initiated by the meta-cleavage of protocatechuic acid that is chromosomally encoded.
Highlights
We report here the draft whole-genome sequence of a fluorene-degrading bacterium, Sphingobium sp. strain LB126
Strain LB126 was kindly provided by Natalie Leys (Belgian Nuclear Research Center, Mol, Belgium) and grown in a mineral medium (4) containing 0.5 g literϪ1 fluorene
The de novo assembly was carried out using the Hierarchical Genome Assembly Process (HGAP)/Quiver protocol in single-molecule real-time (SMRT) Portal version 2.3.0.140936.p5 (5)
Summary
We report here the draft whole-genome sequence of a fluorene-degrading bacterium, Sphingobium sp. strain LB126. Strain LB126 was kindly provided by Natalie Leys (Belgian Nuclear Research Center, Mol, Belgium) and grown in a mineral medium (4) containing 0.5 g literϪ1 fluorene. The whole genome of strain LB126 was sequenced using the PacBio RS II sequencing platform (Pacific Biosciences, Menlo Park, CA). The de novo assembly was carried out using the Hierarchical Genome Assembly Process (HGAP)/Quiver protocol in SMRT Portal version 2.3.0.140936.p5 (5).
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