Abstract
ABSTRACTHere, we report the 4.12-Mb draft genome sequence of Arthrobacter sp. strain 7749, isolated from marine sediment samples of the Antarctic Peninsula, using enriched medium with (RS)-1-(4-phenyl)-ethanol as a carbon source. This genome sequence will provide relevant information for applications in enantioselective alcohol oxidation to improve industrial catalytic processes.
Highlights
Actinobacteria belonging to the genus Arthrobacter constitute a group of Grampositive, chemoorganotrophic, and cosmopolitan soil microorganisms exhibiting nutritional versatility, as demonstrated by their capacity to metabolize numerous substrates, including several toxic compounds, as carbon sources [1,2,3]
Genomic DNA of strain 7749 was extracted from a culture grown for 24 h at 20°C in Luria-Bertani (LB) broth using a Qiagen DNeasy blood and tissue kit, according to the manufacturer’s protocol for Gram-positive bacteria
High-quality genomic DNA was used to construct a library with an approximate insert size of 500 bp using a Nextera XT DNA library preparation kit, according to the manufacturer’s protocol
Summary
Actinobacteria belonging to the genus Arthrobacter constitute a group of Grampositive, chemoorganotrophic, and cosmopolitan soil microorganisms exhibiting nutritional versatility, as demonstrated by their capacity to metabolize numerous substrates, including several toxic compounds, as carbon sources [1,2,3]. Genomic DNA of strain 7749 was extracted from a culture grown for 24 h at 20°C in Luria-Bertani (LB) broth using a Qiagen DNeasy blood and tissue kit (catalog number 69504), according to the manufacturer’s protocol for Gram-positive bacteria. The quantity, purity, and integrity of the genomic DNA were determined using a NanoDrop microvolume spectrophotometer (Thermo Fisher Scientific) and agarose gel ethidiumbromide stain.
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