Abstract
ABSTRACTBotryococcus braunii has long been known as a prodigious producer of liquid hydrocarbon oils that can be converted into combustion engine fuels. This draft genome for the B race of B. braunii will allow researchers to unravel important hydrocarbon biosynthetic pathways and identify possible regulatory networks controlling this unusual metabolism.
Highlights
Botryococcus braunii has long been known as a prodigious producer of liquid hydrocarbon oils that can be converted into combustion engine fuels
A 2 ϫ 300-bp mate-pair library with a 15-kb insert was sequenced to 3ϫ coverage on an Illumina MiSeq
The PacBio data were assembled with FALCON-Unzip, and the resulting sequences were polished using Quiver (8)
Summary
Botryococcus braunii has long been known as a prodigious producer of liquid hydrocarbon oils that can be converted into combustion engine fuels. Genomic DNA was extracted and used to construct four Illumina libraries. A 2 ϫ 250-bp paired-end library constructed from 800-bp fragments was sequenced to 700ϫ coverage on an Illumina HiSeq 2500 platform. 2 ϫ 150-bp mate-pair libraries with 1.5-kb and 4-kb inserts were sequenced to 200ϫ and 150ϫ coverage on a HiSeq 2000.
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