Abstract
ABSTRACTPichia membranifaciens KS47-1 is capable of growing on hydrolysate containing high concentrations of acetate and other growth inhibitors. To reveal the acetate-resistant associate genes of strain KS47-1, we present the 11.4-Mb draft genome sequence.
Highlights
Pichia membranifaciens KS47-1 is capable of growing on hydrolysate containing high concentrations of acetate and other growth inhibitors
We have already isolated an acetate-tolerant yeast, Pichia membranifaciens KS47-1, which is capable of growing on corncob hydrolysate, which strongly inhibits conventional yeast growth, and a medium containing 400 mM acetate
The sequence reads from the paired-end library (400 bp) were initially assembled by Velvet version 1.2.08 using the DDBJ Read Sequence Annotation Pipeline [5]
Summary
Pichia membranifaciens KS47-1 is capable of growing on hydrolysate containing high concentrations of acetate and other growth inhibitors. We have already isolated an acetate-tolerant yeast, Pichia membranifaciens KS47-1, which is capable of growing on corncob hydrolysate, which strongly inhibits conventional yeast growth, and a medium containing 400 mM acetate. We are considering that the strain has great potential for producing biofuel and biomaterial from lignocellulosic feedstocks. Draft genome sequencing was performed to reveal the acetate resistance-associated genes of KS47-1 in the present study.
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