Abstract
We report the draft genome sequences of three vancomycin-susceptible methicillin-resistant Staphylococcus aureus strains. S. aureus strain MV8 is a sequence type 8 (ST-8) staphylococcal cassette chromosome mec element type IV (SCCmec IV) derivative, while the other two strains (S. aureus MM25 and MM61) are ST-5 SCCmec II strains. MM61 is also closely related to the heterogeneous vancomycin-intermediate S. aureus strain MM66.
Highlights
We report the draft genome sequences of the methicillinresistant Staphylococcus aureus (MRSA) strains MM25, MM61, and MV8 (Table 1)
The SmaI chromosomal DNA pulsed-field gel electrophoresis patterns of MM61 and MM66 demonstrated that these strains are clonal [2]
Sequence analysis revealed that MM25 and MM61 are from multilocus sequence type 5 (ST-5) and possess a staphylococcal chromosome cassette mec element type II (SCCmec II), while strain MV8 is from sequence type 8 (ST-8) and harbors SCCmec IV
Summary
We report the draft genome sequences of the methicillinresistant Staphylococcus aureus (MRSA) strains MM25, MM61, and MV8 (Table 1). Genomic libraries were constructed using the Phusion Illumina genomic DNA library protocol (average insert size, 380 bp) and sequenced with the Illumina Genome Analyzer II 90-bp paired-end reads. De novo assemblies were generated using filtered sequence reads utilizing the ABySS version 1.3.7 assembler [3].
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