Abstract
The draft genome sequences of two Salmonella enterica serotype Infantis isolates are reported here. One of the strains was isolated from a western lowland gorilla (Gorilla gorilla gorilla) with colitis. The second strain was isolated from a reptile that inhabited the same premises. Whole-genome sequencing demonstrated that these isolates were not clonal.
Highlights
DNA from both strains was isolated from overnight culture with the Maxwell 16 tissue DNA purification kit using the Maxwell 16 system
The tegu was captured and monitored for Salmonella species shedding by bacteriologic culture
A total of 1,644,295 and 1,624,619 reads were obtained from LPM-ST01 and LPM-ST02, respectively, and were assembled using SPAdes 3.5 software (4), with the “—ion torrent— careful— k 21,33,55,77,97,127” parameters
Summary
DNA from both strains was isolated from overnight culture with the Maxwell 16 tissue DNA purification kit using the Maxwell 16 system (both from Promega, USA). Based on microbiological culture methods, reptiles traditionally have been considered reservoirs of Salmonella species (2). We report the draft genome sequence of an S. enterica serotype Infantis strain (LPM-ST01) isolated from a 13-year-old female western lowland gorilla (Gorilla gorilla gorilla) kept in captivity in Belo Horizonte, Brazil, that developed fatal acute hemorrhagic colitis (3).
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