Draft Genome Sequences of Two Janthinobacteriumlividum Strains, Isolated from Pristine Groundwater Collected from the Oak Ridge Field Research Center.

Xiaoqin Wu,Robert M Walker,Bryson A Cwick,Adam P Arkin,Alexey E Kazakov,Kelly M Wetmore,Pavel S Novichkov,Adam M Deutschbauer,Romy Chakraborty

doi:10.1128/genomea.00582-17

Xiaoqin Wu, Robert M Walker + Show 7 more

Open Access

https://doi.org/10.1128/genomea.00582-17

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Abstract

ABSTRACTWe present here the draft genome sequences of two Janthinobacterium lividum strains, GW456P and GW458P, isolated from groundwater samples collected from a background site at the Oak Ridge Field Research Center. Production of a purple pigment by these two strains was observed when grown on diluted (1/10) LB agar plates.

Highlights

We present here the draft genome sequences of two Janthinobacterium lividum strains, GW456P and GW458P, isolated from groundwater samples collected from a background site at the Oak Ridge Field Research Center
1 ␮g of genomic DNA was fragmented by ultrasonication to an average size of 800 bp with a Covaris S220 focused ultrasonicator
Proteins related to violacein biosynthesis were identified in both strains by comparison with the VioA, VioB, VioC, VioD, and VioE proteins from Janthinobacterium sp

Summary

Introduction

We present here the draft genome sequences of two Janthinobacterium lividum strains, GW456P and GW458P, isolated from groundwater samples collected from a background site at the Oak Ridge Field Research Center. Genomic sequencing libraries of the two J. lividum strains, GW456P and GW458P, were prepared using the NEBNext DNA library prep kit for Illumina (New England Biolabs). 1 ␮g of genomic DNA was fragmented by ultrasonication to an average size of 800 bp with a Covaris S220 focused ultrasonicator. After end-repair, A-tailing, and ligation of the adapter, we size-selected 800-bp products with AMPure XP beads.

Results

Conclusion