Abstract

ABSTRACTOne strain of Trichophyton rubrum CMCC(F)T1i (=CBS 139224) isolated from onychomycosis and one strain of Trichophyton violaceum CMCC(F)T3l (=CBS 141829) isolated from tinea capitis in China were whole-genome sequenced by Illumina/Solexa, while the former was also sequenced by Pacific Biosciences sequencing in parallel.

Highlights

  • One strain of Trichophyton rubrum CMCC(F)T1i isolated from onychomycosis and one strain of Trichophyton violaceum CMCC(F)T3l isolated from tinea capitis in China were whole-genome sequenced by Illumina/Solexa, while the former was sequenced by Pacific Biosciences sequencing in parallel

  • The overall GϩC content of the entire genome is 48.34%, and the N rates are as low as 0.055% for T. rubrum and 47.22% and 0.76% for T. violaceum, respectively

  • A total of 7,170 genes were predicted for T. rubrum and 7,415 for T. violaceum

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Summary

Introduction

One strain of Trichophyton rubrum CMCC(F)T1i (ϭCBS 139224) isolated from onychomycosis and one strain of Trichophyton violaceum CMCC(F)T3l (ϭCBS 141829) isolated from tinea capitis in China were whole-genome sequenced by Illumina/Solexa, while the former was sequenced by Pacific Biosciences sequencing in parallel. Genomic DNA was extracted using the EZNA fungal DNA kit (Omega, USA). For Illumina sequencing, 5-␮g genomic DNA was used in the sequencing library construction. Paired-end libraries with insert sizes of ϳ300 bp were constructed using the AIR paired-end DNA sequencing kit (Bioscientific).

Results
Conclusion

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