Abstract
We report the draft genome sequences of three enteropathogenic Escherichia coli (EPEC) isolates that display the O157 serogroup but do not have the Shiga toxin genes (stx), which are characteristic of O157 enterohemorrhagic E. coli (EHEC). E. coli strain RN587/1 has the O157:H8 serotype and possesses the EAF plasmid characteristic of typical EPEC (J. B. Kaper, J. P. Nataro, and H. L. Mobley, Nat. Rev. Microbiol. 2:123–140, 2004). The other two isolates, strains C844-97 and C639-08, are both O157:H45 and possess the locus of enterocyte effacement (LEE) pathogenicity island; however, they do not contain the EAF plasmid or the stx-carrying phage.
Highlights
Attaching and effacing Escherichia coli (AEEC) is characterized by the presence of the locus of enterocyte effacement (LEE) pathogenicity island [1,2,3] and includes enterohemorrhagic E. coli (EHEC), which expresses Shiga toxins, and typical enteropathogenic E. coli (EPEC), which expresses the EAF plasmid-carried bundle-forming pilus gene [1, 4, 5]
O157:non-H7 E. coli has been isolated from human, animal, and water sources [10, 11], and a recent study demonstrated that the O157-antigen gene cluster is present in E. coli strains with diverse genetic backgrounds rather than being restricted to O157:H7 EHEC [12], suggesting that the serogroup alone is not indicative of increased virulence
C844-97 was isolated in Japan and C639-08 was isolated in Denmark
Summary
Attaching and effacing Escherichia coli (AEEC) is characterized by the presence of the locus of enterocyte effacement (LEE) pathogenicity island [1,2,3] and includes enterohemorrhagic E. coli (EHEC), which expresses Shiga toxins (encoded by stx1 and/or stx2), and typical enteropathogenic E. coli (EPEC), which expresses the EAF plasmid-carried bundle-forming pilus gene (bfp) [1, 4, 5]. O157:H45 EPEC isolates with the genotype bfpϩ have been previously associated with an outbreak of diarrhea in Japan [9]. O157:non-H7 E. coli has been isolated from human, animal, and water sources [10, 11], and a recent study demonstrated that the O157-antigen gene cluster is present in E. coli strains with diverse genetic backgrounds rather than being restricted to O157:H7 EHEC [12], suggesting that the serogroup alone is not indicative of increased virulence.
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