Abstract
ABSTRACTAcinetobacter baumannii is an important opportunistic pathogen in global health care settings. Its dissemination and multidrug resistance pose an issue with treatment and outbreak control. Here, we present draft genome assemblies of six multidrug-resistant clinical strains of A. baumannii isolated from patients admitted to one of two major hospitals in Kuwait.
Highlights
Acinetobacter baumannii is an important opportunistic pathogen in global health care settings
Six clinical isolates of A. baumannii, three isolates each from Al-Sabah Hospital (KUSSH13, KUSSH35, and KUSSH36) and Farwaniya Hospital (KUFAR40, KUFAR42, and KUFAR44) (Table 1), were grown on blood agar plates, and single colonies were suspended in nuclease-free water
The bacterial suspensions were heated at 95°C for 25 min, and DNA was purified using the QIAamp DNA minikit (Qiagen, Hilden, Germany) and quantified using the Qubit double-stranded DNA broad-range assay (Thermo Fisher Scientific)
Summary
Acinetobacter baumannii is an important opportunistic pathogen in global health care settings. Six clinical isolates of A. baumannii, three isolates each from Al-Sabah Hospital (KUSSH13, KUSSH35, and KUSSH36) and Farwaniya Hospital (KUFAR40, KUFAR42, and KUFAR44) (Table 1), were grown on blood agar plates, and single colonies were suspended in nuclease-free water.
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