Draft Genome Sequences of Six Different Staphylococcus epidermidis Clones, Isolated Individually from Preterm Neonates Presenting with Sepsis at Edinburgh's Royal Infirmary.

P Walsh,C Smith,T Manning,X Lu,B Kelly,P Ghazal,R D Sleator,A O’Driscoll,M Bekaert,J Carroll,P Dickinson,K Templeton

doi:10.1128/genomea.00471-15

Abstract

Herein, we report the draft genome sequences of six individual Staphylococcus epidermidis clones, cultivated from blood taken from different preterm neonatal sepsis patients at the Royal Infirmary, Edinburgh, Scotland, United Kingdom.

Highlights

Staphylococcus epidermidis is a Gram-positive bacterium naturally found on human skin [1] and an important opportunistic pathogen linked with neonatal blood sepsis [2,3,4,5]
As part of the ClouDx-i project consortium, we aim to extend our knowledge of currently circulating pathogenic strains linked with neonatal blood sepsis to inform the continued development of new and improved molecular diagnostic assays [11]
The predicted open reading frames (ORFs) were compared to the Uniprot Trembl database [15] using BLASTp. Samples were handled in accordance with local ethical approval by the ethics committees of the NHS Lothian SAHSC Bioresource and NHS R&D office (project 2011/R/NE/01) and the HSS BioResource (request 13/ES/0126)

Summary

Introduction

Staphylococcus epidermidis is a Gram-positive bacterium naturally found on human skin [1] and an important opportunistic pathogen linked with neonatal blood sepsis [2,3,4,5]. Rapid detection of blood sepsis and characterization of the causative pathogen are critical first steps to enable appropriate treatment and improved prognostic outcomes [8,9,10]. As part of the ClouDx-i project consortium, we aim to extend our knowledge of currently circulating pathogenic strains linked with neonatal blood sepsis to inform the continued development of new and improved molecular diagnostic assays [11].

Objectives

Results

Conclusion