Abstract
A mixed culture of Pseudomonas fluorescens BS2 and Pusillimonas noertemannii BS8 degraded poly-γ-d-glutamic acid; when the 2 strains were cultured separately, no hydrolytic activity was apparent. Here we report the draft genome sequences of both soil isolates.
Highlights
A mixed culture of Pseudomonas fluorescens BS2 and Pusillimonas noertemannii BS8 degraded poly-␥-D-glutamic acid; when the 2 strains were cultured separately, no hydrolytic activity was apparent
Anthrax is an excellent candidate for this approach: the inhalational form of the infection requires prompt therapy, and the causative agent, Bacillus anthracis, invariably expresses a unique poly-␥-D-glutamic acid (PDGA) capsule that is necessary for optimal pathogenesis [3]
The poly␥-glutamate-specific capsule depolymerase (CapD) produced by B. anthracis removes the capsule from the surface of the anthrax bacillus [4] and can protect against anthrax infection [5] but is markedly unstable [6] and unlikely to be useful for therapeutic development
Summary
A mixed culture of Pseudomonas fluorescens BS2 and Pusillimonas noertemannii BS8 degraded poly-␥-D-glutamic acid; when the 2 strains were cultured separately, no hydrolytic activity was apparent. Experimental infections caused by bacteria that elaborate a polysaccharide or polypeptide capsule can be resolved by administration of capsule hydrolases that strip away the protective capsular layer from the bacterial surface [1, 2]. Anthrax is an excellent candidate for this approach: the inhalational form of the infection requires prompt therapy, and the causative agent, Bacillus anthracis, invariably expresses a unique poly-␥-D-glutamic acid (PDGA) capsule that is necessary for optimal pathogenesis [3].
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