Abstract
ABSTRACTListeria monocytogenes has caused listeriosis outbreaks linked to soft cheese. Here, we report the draft genome sequences of seven L. monocytogenes isolates from two possibly related outbreaks caused by soft cheese products in Washington State.
Highlights
Listeria monocytogenes is a ubiquitous, Gram-positive, non-spore-forming, rod-shaped, motile aerobic, and facultatively anaerobic bacterium [1]
The PCR serogroups were determined at the Institut Pasteur multilocus sequence typing (MLST) database
MLST analysis indicated that all the isolates belonged to lineage I, sequence type 663, clonal complex 663, and PCR serogroup IVb
Summary
Listeria monocytogenes is a ubiquitous, Gram-positive, non-spore-forming, rod-shaped, motile aerobic, and facultatively anaerobic bacterium [1]. Listeria DNA was extracted using a DNeasy blood and tissue kit (Qiagen, Valencia, CA) and prepared with an Illumina Nextera XT kit. The 250-bp paired-end sequencing was performed using an Illumina MiSeq sequencer. Raw sequencing reads were quality trimmed using Trimmomatic (version 0.36) [5].
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