Abstract
ABSTRACTWe present draft genome sequences of five Enterococcus species from patients suspected of Clostridium difficile infection. Genome completeness was confirmed by presence of bacterial orthologs (97%). Gene searches using Hidden-Markov models revealed that the isolates harbor between seven and 11 genes involved in antibiotic resistance to tetracyclines, beta-lactams, and vancomycin.
Highlights
Numerous reports link microbial compositional changes in the human gut microbiota to diverse disease states ranging from inflammatory illness to psychiatric conditions [1,2,3,4]
We collected fecal samples from patients suspected of being infected by C. difficile that presented aqueous diarrhea associated with antimicrobial drug intake
DNA was quantified in a fluorimeter (Qubit; Invitrogen) and its integrity was checked by agarose gel electrophoresis
Summary
Numerous reports link microbial compositional changes (dysbiosis) in the human gut microbiota to diverse disease states ranging from inflammatory illness to psychiatric conditions [1,2,3,4]. We collected fecal samples from patients suspected of being infected by C. difficile that presented aqueous diarrhea associated with antimicrobial drug intake. We plated samples on blood agar medium (Merck; anaerobic conditions) and grew colonies in Brucella broth at 37°C without agitation [7]. For DNA extraction, we used the Wizard genomic DNA purification kit (Promega) following the manufacturer’s instructions.
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