Abstract
ABSTRACTCarboxydothermus spp. are some of the most studied carbon monoxide–oxidizing anaerobic thermophiles. For further investigation into the carbon monoxide metabolism of Carboxydothermus spp., we report here the draft genome sequences of the hydrogenogenic carboxydotrophs Carboxydothermus pertinax (2.47 Mb; G+C content, 40.7%) and C. islandicus (2.39 Mb; G+C content, 42.0%).
Highlights
Carboxydothermus spp. are some of the most studied carbon monoxide– oxidizing anaerobic thermophiles
We identified four CO dehydrogenase (CODH) gene clusters [8] in C. pertinax and five CODH gene clusters [8] in C. islandicus
The functions of the five CODHs in C. hydrogenoformans have been predicted according to empirical evidence and/or the genomic contexts of their genes [8, 14, 15]: CODH-I for energy conversion conjugated with energy-converting hydrogenase (ECH); CODH-II for NAD (P) H generation; CODH-III for carbon fixation in the Wood–Ljungdahl pathway; CODH-IV for oxidative stress response; and CODH-V for unknown function
Summary
Carboxydothermus spp. are some of the most studied carbon monoxide– oxidizing anaerobic thermophiles. Genomic DNA of C. pertinax and C. islandicus, extracted using previously described NaOH methods [9], were subjected to sequencing with the Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) using the 2 ϫ 150-bp paired-end approach, generating 3,839,951 and 6,663,869 paired-end reads, respectively. High-quality reads (Phred score above Q20 for 80% of the bases) were assembled into contigs using Velvet version 1.2.07 or version 1.2.10 software [10].
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