Abstract
Draft genome sequences of two Campylobacterales (Sulfurospirillum sp. strain SCADC and Sulfuricurvum sp. strain MLSB [Mildred Lake Settling Basin]) were obtained by taxonomic binning of metagenomes originating from an oil sands tailings pond. Both genomes contain soxABXYZ genes involved in sulfur oxidation, highlighting their potential roles in sulfur cycling in oil sands tailings ponds.
Highlights
Draft genome sequences of two Campylobacterales (Sulfurospirillum sp. strain SCADC and Sulfuricurvum sp. strain MLSB [Mildred Lake Settling Basin]) were obtained by taxonomic binning of metagenomes originating from an oil sands tailings pond
Contigs assembled from the MLSB metagenome and from the metagenome of SCADC were binned using sequence homology and composition-based methods, including sequence coverage, GC content, and contig length, followed by tetranucleotide frequency and analysis of principal components
BLASTn comparison of 16S rRNA genes in the SCADC Campylobacterales bin indicated that the draft genome is closely (99%) related to Sulfurospirillum multivorans DSM 12446 (CP007201)
Summary
Draft genome sequences of two Campylobacterales (Sulfurospirillum sp. strain SCADC and Sulfuricurvum sp. strain MLSB [Mildred Lake Settling Basin]) were obtained by taxonomic binning of metagenomes originating from an oil sands tailings pond. The metagenome of MLSB (Mildred Lake Settling Basin), an oil sands tailings pond located in northern Alberta, Canada, was obtained by isolating total DNA for high-throughput sequencing using Illumina-HiSeq. Reads were subjected to quality control and sequence assembly using CLC Genomics Workbench version 7 (CLC-Bio, USA). Two Campylobacterales-associated genomic bins were recovered and subjected to sequence decontamination as previously described [6].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
