Draft Genome Sequences from Cyclospora cayetanensis Oocysts Purified from a Human Stool Sample.

Yvonne Qvarnstrom,Vishal Nayak,Mili Sheth,Ganesh Srinivasamoorthy,Fernanda S Nascimento,Michael J Arrowood,Delynn M Moss,Barbara L Herwaldt,Henry S Bishop,Wen Li,Yuping Wei-Pridgeon

doi:10.1128/genomea.01324-15

Abstract

The parasite Cyclospora cayetanensis causes foodborne diarrheal illness. Here, we report draft genome sequences obtained from C. cayetanensis oocysts purified from a human stool sample. The genome assembly consists of 865 contigs with a total length of 44,563,857 bases. These sequences can facilitate the development of subtyping tools to aid outbreak investigations.

Highlights

The parasite Cyclospora cayetanensis causes foodborne diarrheal illness
Cyclosporiasis is not thought to be endemic in the United States: the two established risk factors for U.S cases are international travel to regions where cyclosporiasis is endemic and the consumption of contaminated fresh produce imported from such regions [1, 2]
Outbreak investigations and studies of the epidemiology of cyclosporiasis have been hampered by multiple factors, including the lack of laboratory methods for strain subtyping and for sensitive detection of oocysts in food and environmental samples

Summary

Introduction

The parasite Cyclospora cayetanensis causes foodborne diarrheal illness. Here, we report draft genome sequences obtained from C. cayetanensis oocysts purified from a human stool sample. Cyclospora cayetanensis is a coccidian parasite that causes cyclosporiasis. Humans become infected by ingesting food or water contaminated with mature (sporulated) oocysts. U.S foodborne outbreaks have been detected almost every year since the mid-1990s (http://www.cdc.gov/parasites/cyclosporiasis /outbreaks/foodborneoutbreaks.html [1]).

Objectives

Results

Conclusion