Abstract
The draft genome of an uncultivated bacterium affiliated with the Peptococcaceae was reconstructed by co-assembling Illumina MiSeq sequences from three single cells sorted by microfluidics from two methanogenic alkane-degrading cultures. Peptococcaceae SCADC (short-chain alkane-degrading culture) may be genetically capable of anaerobic alkane activation by fumarate addition in the absence of sulfate.
Highlights
The draft genome of an uncultivated bacterium affiliated with the Peptococcaceae was reconstructed by co-assembling Illumina MiSeq sequences from three single cells sorted by microfluidics from two methanogenic alkane-degrading cultures
We previously described a methanogenic SCADC enriched from an oil sands tailings pond (1)
Two cells from SCADC and one from iso-alkane-degrading culture (ISODC) were subjected to multiple displacement amplification, sequenced as single-amplified genomes (SAGs) using Illumina MiSeq, and individually assembled de novo using SPAdes version 3.1 (2) with a read correction module and k-mer sizes of 21, 33, 45, 55, 77, 99, and 127
Summary
The draft genome of an uncultivated bacterium affiliated with the Peptococcaceae was reconstructed by co-assembling Illumina MiSeq sequences from three single cells sorted by microfluidics from two methanogenic alkane-degrading cultures. We previously described a methanogenic SCADC (shortchain alkane-degrading culture) enriched from an oil sands tailings pond (1). Subsamples of SCADC and a methanogenic iso-alkane-degrading culture
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