Abstract
ABSTRACTA draft genome sequence of a neutrophilic halophile capable of thiocyanate degradation, Thiohalobacter thiocyanaticus FOKN1, was determined using a PacBio RSII sequencer. A 3.23-Mb circular genome sequence was assembled, in which 3,026 gene-coding sequences, 45 tRNAs, and 1 rrn operon were annotated.
Highlights
A draft genome sequence of a neutrophilic halophile capable of thiocyanate degradation, Thiohalobacter thiocyanaticus FOKN1, was determined using a PacBio RSII sequencer
The culture was aerobically incubated at 30°C, and a thiocyanate-degrading neutrophilic halophile strain, FOKN1, was isolated from the enrichment culture by the serial dilution method
The full-length 16S rRNA gene sequence (1,504 bp) from the genome was subjected to BLASTn analysis, and the 16S rRNA gene sequence showed 99.1% similarity with that of the thiocyanate-degrading neutrophilic halophile Thiohalobacter thiocyanaticus HRh1, previously isolated from
Summary
A draft genome sequence of a neutrophilic halophile capable of thiocyanate degradation, Thiohalobacter thiocyanaticus FOKN1, was determined using a PacBio RSII sequencer. We initially enriched thiocyanate-degrading neutrophilic halophiles from activated sludge by inoculating the biomass into an inorganic medium containing thiocyanate (per liter, 320 mg of sodium thiocyanate, 1,650 mg of ammonium chloride, 650 mg of sodium hydrogen carbonate, 29.2 mg of disodium phosphate, 0.001% [wt/vol] yeast extract, and 60% [vol/vol] seawater [pH 7.0 to 7.5]). The culture was aerobically incubated at 30°C, and a thiocyanate-degrading neutrophilic halophile strain, FOKN1, was isolated from the enrichment culture by the serial dilution method.
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