Abstract
ABSTRACTA draft genome sequence of Halobacteriovorax sp. strain JY17 was assembled from a metagenomic data set. The 3.47-Mbp genome of this unusual predatory bacterium contains 3,263 protein-coding sequences, 33 tRNAs, and 2 copies each of the 16S, 23S, and 5S rRNA genes. This is only the third sequenced representative of this genus.
Highlights
A draft genome sequence of Halobacteriovorax sp. strain JY17 was assembled from a metagenomic data set
Raw reads from three individual samples were pre-processed using BBTools [6] and coassembled using metaSPAdes [7]
The resulting bins were manually refined to reduce contamination using Anvi’o [10]; briefly, for each bin, contigs were clustered on the basis of GC% by Euclidean distance and Ward’s linkage clustering followed by visualization of the resulting dendrograms and manual removal of any outliers
Summary
A draft genome sequence of Halobacteriovorax sp. strain JY17 was assembled from a metagenomic data set. Raw reads from three individual samples were pre-processed using BBTools [6] and coassembled using metaSPAdes [7]. Contigs were taxonomically assigned using Kaiju [8] and binned on the basis of GC% and differential coverage using CONCOCT [9]. The resulting bins were manually refined to reduce contamination using Anvi’o [10]; briefly, for each bin, contigs were clustered on the basis of GC% by Euclidean distance and Ward’s linkage clustering followed by visualization of the resulting dendrograms and manual removal of any outliers.
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