Abstract
ABSTRACTWe describe here the draft genome sequence of the cyanobacterium Tychonema bourrellyi, assembled from a metagenome of a nonaxenic culture. The strain (FEM_GT703) was isolated from a freshwater sample taken from Lake Garda, Italy. The draft genome sequence represents the first assembled T. bourrellyi strain.
Highlights
We describe here the draft genome sequence of the cyanobacterium Tychonema bourrellyi, assembled from a metagenome of a nonaxenic culture
A single filament of Tychonema was isolated under the microscope and, after being washed repeatedly with Z8 Cellstar medium (Greiner Bio-One GmbH, Germany), was incubated at 20°C at constant-light illumination (3)
The Tychonema culture was filtered through 0.22-m filters, and the PowerWater DNA isolation kit (Qiagen) was used to extract the DNA
Summary
We describe here the draft genome sequence of the cyanobacterium Tychonema bourrellyi, assembled from a metagenome of a nonaxenic culture. We reconstructed the draft genome of T. bourrellyi from a nonaxenic culture. The culture originated from a water sample collected in summer 2014 by vertical tows from 20 to 30 m to the surface with a plankton net at Lake Garda.
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