Abstract
ABSTRACTWe sequenced a naturally competent bacterial isolate, WY10, cultured from a Wyoming soil sample. Sequence analysis revealed that WY10 is a novel strain of Bacillus simplex. To our knowledge, WY10 is the first B. simplex strain to be characterized as naturally competent for DNA uptake by transformation.
Highlights
We sequenced a naturally competent bacterial isolate, WY10, cultured from a Wyoming soil sample
DNA was extracted from a WY10 overnight culture with phenol-chloroform, processed into sequencing libraries (Nextera XT kit; Illumina), and sequenced using 2 ϫ 250 paired-end reads (MiSeq; Illumina)
We recently reported that certain bacteriophages, dubbed “superspreaders,” do not efficiently degrade bacterial plasmids during infection and instead release intact plasmid DNA upon lysis, thereby promoting horizontal gene transfer by transformation (3)
Summary
We sequenced a naturally competent bacterial isolate, WY10, cultured from a Wyoming soil sample. DNA was extracted from a WY10 overnight culture with phenol-chloroform, processed into sequencing libraries (Nextera XT kit; Illumina), and sequenced using 2 ϫ 250 paired-end reads (MiSeq; Illumina). The resulting 4,066,400 paired-end reads were trimmed of Illumina adapters and human-like sequences with Trimmomatic (4) and Deconseq (5), respectively.
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