Draft Genome Sequence of the Mycobacterium tuberculosis Complex Pathogen M. mungi, Identified in a Banded Mongoose (Mungos mungo) in Northern Botswana.

Kathleen A Alexander,Michelle H Larsen,Patrick M Camp,Suelee Robbe-Austerman,Tod P Stuber

doi:10.1128/genomea.00471-16

Abstract

Mycobacterium mungi, a Mycobacterium tuberculosis complex pathogen, has emerged in banded mongoose in northern Botswana and Northwest Zimbabwe. The pathogen is transmitted through infected secretions used in olfactory communication behavior (K. A. Alexander, C. E. Sanderson, M. H. Larsen, S. Robbe-Austerman, M. C. Williams, and M. V. Palmer, mBio 7(3):e00281-16, 2016, http://dx.doi.org/10.1128/mBio.00281-16). We announce here the draft genome sequence of this emerging pathogen.

Highlights

In northern Botswana, banded mongoose have been the subject of intensive study since the pathogen emerged in 1999 (IACUC 13-164-FIW and Botswana Ministry of Environment, Wildlife and Tourism EWT 8/36/4 XXVI [24])
Because this Mycobacterium tuberculosis complex (MTBC) pathogen has not been successfully cultivated in vitro, wholegenome shotgun sequencing was performed directly from affected tissue. Several tissues from this mongoose were screened with a real-time IS6110 MTBC PCR (6), and a small portion (3 g) of the severely diseased liver with the lowest threshold cycle (CT) value (13.8) was homogenized thoroughly using a gentleMACS M tube (Milteny Biotec, San Diego, CA, USA)
We report here the first draft genome of the only known MTBC species that has not been successfully cultured in vitro

Summary

Introduction

In northern Botswana, banded mongoose have been the subject of intensive study since the pathogen emerged in 1999 (IACUC 13-164-FIW and Botswana Ministry of Environment, Wildlife and Tourism EWT 8/36/4 XXVI [24]). Because this Mycobacterium tuberculosis complex (MTBC) pathogen has not been successfully cultivated in vitro, wholegenome shotgun sequencing was performed directly from affected tissue. Several tissues from this mongoose were screened with a real-time IS6110 MTBC PCR (6), and a small portion (3 g) of the severely diseased liver with the lowest threshold cycle (CT) value (13.8) was homogenized thoroughly using a gentleMACS M tube (Milteny Biotec, San Diego, CA, USA).

Results

Conclusion