Abstract
ABSTRACTWe announce here the draft genome assembly of Flavobacterium columnare CSF-298-10, a strain isolated from an outbreak of columnaris disease at a commercial trout farm in Hagerman Valley, Idaho, USA. The complete genome consists of 13 contigs totaling 3,284,579 bp, with an average G+C content of 31.5% and 2,933 predicted coding genes.
Highlights
We announce here the draft genome assembly of Flavobacterium columnare CSF-298-10, a strain isolated from an outbreak of columnaris disease at a commercial trout farm in Hagerman Valley, Idaho, USA
F. columnare CSF-298-10 was cultured in tryptone yeast extract salt broth medium at 30°C and 150 rpm (Innova44 incubator, New Brunswick Scientific)
The average GϩC content is 31.5%, which is consistent with the other F. columnare genomovar I strains previously sequenced, namely, ATCC 49512 (31.5%) [10] and Pf1 (31.58%) [11], and is slightly higher than the GϩC content of the previously sequenced genomovar II strains C#2 (30.97%) [12] and 94-081 (30.8%) [13]
Summary
We announce here the draft genome assembly of Flavobacterium columnare CSF-298-10, a strain isolated from an outbreak of columnaris disease at a commercial trout farm in Hagerman Valley, Idaho, USA. F. columnare CSF-298-10 was cultured in tryptone yeast extract salt broth medium at 30°C and 150 rpm (Innova44 incubator, New Brunswick Scientific). Genomic DNA was isolated from 1.5 mL of overnight culture using the MasterPure Gram-positive DNA purification kit (Epicentre), and sent away for Illumina HiSeq sequencing at Arizona Research Labs (Tucson, AZ, USA). Quality filtering and trimming were performed using bcl2fastq conversion software version 1.8.2 and Trimmomatic version 0.30 with parameters TRAILING:3.
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