Abstract
Here, we present the draft genome sequence of the dimorphic ascomycete yeast Yarrowia lipolytica strain W29 (ATCC 20460). Y. lipolytica is a commonly employed model for the industrial production of lipases, small molecules, and more recently for its ability to accumulate lipids.
Highlights
We present the draft genome sequence of the dimorphic ascomycete yeast Yarrowia lipolytica strain W29 (ATCC 20460)
We sequenced genomic DNA from six mutants derived from UV mutagenesis of Yarrowia lipolytica strain W29 (ATCC 20460) using a whole-genome shotgun sequencing approach on an Illumina HiSeq instrument
The resulting assembly had a size of 20.3 Mb (N50, 125,743 bp; Nmax, 356,851 bp; median coverage, 148.2ϫ) and GϩC content of 49.0%
Summary
We present the draft genome sequence of the dimorphic ascomycete yeast Yarrowia lipolytica strain W29 (ATCC 20460). We sequenced genomic DNA from six mutants derived from UV mutagenesis of Yarrowia lipolytica strain W29 (ATCC 20460) using a whole-genome shotgun sequencing approach on an Illumina HiSeq instrument. Ten million paired-end 150-bp reads with an average insert size of 243 Ϯ 28 bp (standard deviation) from each strain (60 million reads total) were assembled into a consensus sequence of 369 contigs using Velvet version 1.2.10 [1].
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