Abstract
We report the draft genome sequence of Pseudomonas sp. nov. H2, isolated from creek sediment in Moscow, ID, USA. The strain is most closely related to Pseudomonas putida. However, it has a slightly smaller genome that appears to have been impacted by horizontal gene transfer and poorly maintains IncP-1 plasmids.
Highlights
Genomic DNA was isolated using a GenElute bacterial genomic DNA kit (Sigma-Aldrich) as per manufacturer’s instructions
The Genomic DNA (gDNA) was sequenced using a whole-genome shotgun approach with paired 150 bp reads generated on MiSeq (Illumina) at the IBEST Genomics Resources Core at the University of Idaho, ID, USA
Strain H2 was identified as a potential new Pseudomonas species using an in-house, four-gene multilocus sequence analysis (MLSA) scheme
Summary
Genomic DNA (gDNA) was isolated using a GenElute bacterial genomic DNA kit (Sigma-Aldrich) as per manufacturer’s instructions. The strain was isolated from creek sediment in Moscow, ID, as a transconjugant after a plate mating of a sediment sample with an auxotrophic donor of the IncP-1 plasmid pB10. Based on its 16S rRNA sequence, it was provisionally called P. putida H2.
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