Abstract
ABSTRACTProvidencia stuartii is frequently associated with nosocomial outbreaks and displays intrinsic resistance to many commonly used antimicrobials. We report here the draft genome sequence of a P. stuartii strain carrying acquired resistance genes conferring panresistance to cephalosporins (blaSHV-5 and blaVEB-1), carbapenems (blaVIM-1), and aminoglycosides (rmtB) involved in an outbreak in Greek hospitals.
Highlights
Providencia stuartii has emerged as an important nosocomial pathogen responsible for urinary tract infections in patients with indwelling urinary catheters, hospitalacquired pneumonia, bloodstream infections, and sepsis (1), with significant impact on patient morbidity, mortality, treatment, and management costs (2)
The draft genome sequence of PS71 consists of 4,411,042 bp with 41.75% average GϩC content
Provisional annotation carried out using the Prokka algorithm (9) identified at least 4,026 coding sequences (CDSs), including 76 tRNAs and eight rRNAs
Summary
Providencia stuartii has emerged as an important nosocomial pathogen responsible for urinary tract infections in patients with indwelling urinary catheters, hospitalacquired pneumonia, bloodstream infections, and sepsis (1), with significant impact on patient morbidity, mortality, treatment, and management costs (2). Genomic DNA was extracted and subjected to whole-genome sequencing with the Illumina MiSeq platform (Illumina, Inc., San Diego, CA), producing 2 ϫ 250-bp pairedend reads, generating a total of 843,412 reads with a 475 bp average length. The Trimmomatic algorithm (version 0.36) (4) was used to trim all the generated reads and their quality assessed with in-house scripts using BedTools (version 2.25.0) (5), BWAmem (version 2) (6), and SAMtools (version 1.3.1) (7) algorithms.
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