Draft Genome Sequence of Methylobacterium sp. Strain V23, Isolated from Accretion Ice of the Antarctic Subglacial Lake Vostok.

Amanda Sapp,Maximiliano Sánchez-Lamas,Sebastián Klinke,Jimena Rinaldi,Lisandro H Otero,Fernando A Goldbaum,Hernán R Bonomi,Giuliano T Antelo,Brent C Christner,Emiliano D Primo,José C Huguet-Tapia

doi:10.1128/genomea.00145-18

Abstract

ABSTRACTHere, we report the draft genome sequence of Methylobacterium sp. strain V23, a bacterium isolated from accretion ice of the subglacial Lake Vostok (3,592 meters below the surface). This genome makes possible the study of ancient and psychrophilic genes and proteins from a subglacial environment isolated from the surface for at least 15 million years.

Highlights

We report the draft genome sequence of Methylobacterium sp. strain V23, a bacterium isolated from accretion ice of the subglacial Lake Vostok (3,592 meters below the surface)
Wholegenome de novo sequencing was performed with 100-bp paired-end reads using the Illumina HiSeq 4000 platform (Macrogen, Inc.)
The DNA library was prepared using a TruSeq DNA PCR-free kit according to the instructions in the TruSeq DNA PCR-free sample preparation guide (Macrogen, Inc.)

Summary

Introduction

We report the draft genome sequence of Methylobacterium sp. strain V23, a bacterium isolated from accretion ice of the subglacial Lake Vostok (3,592 meters below the surface). Isolate V23 was cultured as previously reported [3], and its genomic DNA was isolated using the UltraClean microbial DNA isolation kit (Mo Bio, Inc., USA). Wholegenome de novo sequencing was performed with 100-bp paired-end reads using the Illumina HiSeq 4000 platform (Macrogen, Inc.). The DNA library was prepared using a TruSeq DNA PCR-free kit according to the instructions in the TruSeq DNA PCR-free sample preparation guide (Macrogen, Inc.).

Results

Conclusion