Draft Genome Sequence of Methanoculleus sediminis S3FaT, a Hydrogenotrophic Methanogen Isolated from a Submarine Mud Volcano in Taiwan.

Sheng-Chung Chen,Sue-Yao Wu,Mei-Fei Chen,Chieh-Yin Weng,Mei-Chin Lai

doi:10.1128/genomea.00308-16

Abstract

Here, we announce the genome sequence of Methanoculleus sediminis S3FaT (DSM 29354T), a strict anaerobic methanoarchaeon, which was isolated from sediments near the submarine mud volcano MV4 located offshore in southwestern Taiwan. The 2.49-Mb genome consists of 2,459 predicted genes, 3 rRNAs, 48 tRNAs, and 1 ncRNA. The sequence of this novel strain may provide more information for species delineation and the roles that this strain plays in the unique marine mud volcano habitat.

Highlights

Methanoculleus sediminis S3FaT, a mesophilic and hydrogenotrophic methanogen, was isolated from sediments near the submarine mud volcano MV4 located offshore in southwestern Taiwan and described as a novel species within the genus Methanoculleus (1)
Gene annotations were performed by using the NCBI Prokaryotic Genome Automatic Annotation Pipeline (PGAAP); 2,459 predicted genes, 3 rRNAs, 48 tRNAs, and 1 ncRNA were obtained
The genome contains the genes of formate dehydrogenase, which is essential for formate utilization for methane production and growth of Methanoculleus sediminis S3FaT

Summary

Introduction

Methanoculleus sediminis S3FaT, a mesophilic and hydrogenotrophic methanogen, was isolated from sediments near the submarine mud volcano MV4 located offshore in southwestern Taiwan and described as a novel species within the genus Methanoculleus (1). The genus Methanoculleus currently comprises 11 characterized and valid species: M. marisnigri JR1T (2), M. submarinus Nankai-1T (3), M. chikugoensis MG62T (4), M. thermophiles CR-1T (5), M. palmolei INSLUZT (6), M. bourgensis MS2T (7), M. receptaculi ZC-2T (8), M. hydrogenitrophicus HCT (9), M. horonobensis T10T (10), M. taiwanensis CYW4T (11), and M. sediminis S3FaT (1). The sources of these Methanoculleus spp. are truly diverse, including marine sediments, wastewater plants, wetlands, oil fields, and paddy fields.

Results

Conclusion