Draft Genome Sequence of Magnetospirillum sp. Strain 15-1, a Denitrifying Toluene Degrader Isolated from a Planted Fixed-Bed Reactor.

Ingrid Meyer-Cifuentes,Stefan Fiedler,Hermann J Heipieper,Ines Mäusezahl,Uwe Kappelmeyer,Jochen A Müller

doi:10.1128/genomea.00764-17

Ingrid Meyer-Cifuentes, Stefan Fiedler + Show 4 more

Open Access

https://doi.org/10.1128/genomea.00764-17

Copy DOI

Abstract

ABSTRACTHere, we report the draft genome sequence of Magnetospirillum sp. 15-1. This strain was isolated from a planted fixed-bed reactor based on its ability to degrade toluene under anaerobic conditions. The genome assembly consists of 5.4 Mb in 28 contigs and 5,095 coding sequences containing the genes involved in anaerobic toluene degradation.

Highlights

We report the draft genome sequence of Magnetospirillum sp. 15-1
We present the draft genome sequence of Magnetospirillum sp.15-1, a nonmagnetic strain isolated from a planted fixed-bed reactor (PFR) continuously fed with toluene [6]
The draft genome sequence of Magnetospirillum sp. 15-1 consists of 5,422,505 bp with an average GϩC content of 65.6%

Summary

Introduction

We report the draft genome sequence of Magnetospirillum sp. 15-1. This strain was isolated from a planted fixed-bed reactor based on its ability to degrade toluene under anaerobic conditions. We present the draft genome sequence of Magnetospirillum sp.15-1, a nonmagnetic strain isolated from a planted fixed-bed reactor (PFR) continuously fed with toluene [6]. Sequencing was carried out using the 454-GS Junior system (Roche Applied Science) and MiSeq instrument (Illumina). Illumina libraries were paired-end sequenced with a maximum 300-bp read length (MiSeq reagent kit version 3, 600 cycles, Illumina). De novo assembly was conducted with the A5-miseq pipeline [7] and Geneious assembler (http://www.geneious.com) [8], generating 527 contigs initially.

Results

Conclusion