Abstract
Brevibacillus thermoruber strain 423 is a Gram-positive, spore-forming, aerobic, and thermophilic bacterium that produces mannogalactoglucan exopolysaccharide (EPS). We report the draft genome sequence of B. thermoruber 423, which will accelerate research on the cellular organization of thermophilic bacteria, as well as the rational design and optimization of EPS production.
Highlights
Brevibacillus thermoruber strain 423 is a Gram-positive, spore-forming, aerobic, and thermophilic bacterium that produces mannogalactoglucan exopolysaccharide (EPS)
Given the need for a good EPS-producing thermophile that could be used as a model organism to study the biological mechanisms and wholegenome organization of EPS-producing thermophilic bacteria, Brevibacillus thermoruber strain 423, isolated from a hot spring in southwest Bulgaria and found to excrete high titers of EPS outperforming reported yields (3), was sequenced via next-generation sequencing (NGS) technology
Whole-genome sequencing of B. thermoruber 423 was performed via Illumina HiSeq 2000 technology
Summary
Brevibacillus thermoruber strain 423 is a Gram-positive, spore-forming, aerobic, and thermophilic bacterium that produces mannogalactoglucan exopolysaccharide (EPS). Given the need for a good EPS-producing thermophile that could be used as a model organism to study the biological mechanisms and wholegenome organization of EPS-producing thermophilic bacteria, Brevibacillus thermoruber strain 423, isolated from a hot spring (temperature, 59°C; pH 6.5) in southwest Bulgaria and found to excrete high titers of EPS outperforming reported yields (3), was sequenced via next-generation sequencing (NGS) technology. Whole-genome sequencing of B. thermoruber 423 was performed via Illumina HiSeq 2000 technology.
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