Abstract
Escherichia coli strain VKPM B-10182, obtained by chemical mutagenesis from E. coli strain ATCC 9637, produces cephalosporin acid synthetase employed in the synthesis of β-lactam antibiotics, such as cefazolin. The draft genome sequence of strain VKPM B-10182 revealed 32 indels and 1,780 point mutations that might account for the improvement in antibiotic synthesis that we observed.
Highlights
Escherichia coli strain VKPM B-10182, obtained by chemical mutagenesis from E. coli strain ATCC 9637, produces cephalosporin acid synthetase employed in the synthesis of -lactam antibiotics, such as cefazolin
Escherichia coli strain VKPM B-10182 produces a peptidohydrolase specific to the derivatives of phenylacetic acid, tetrazolylacetic acid, and thienylacetic acid. This enzyme, originally named cephalosporin acid synthetase, is able to synthesize antibiotics belonging to -lactam acids, especially to cephalosporin acids, such as cefazolin
It is very promising for application in the industrial manufacturing of corresponding antibiotics by biocatalytic synthesis [1,2,3] and is identical to penicillin G acylase (PGA), which is involved in benzylpenicillin hydrolysis [4, 5]
Summary
Escherichia coli strain VKPM B-10182, obtained by chemical mutagenesis from E. coli strain ATCC 9637, produces cephalosporin acid synthetase employed in the synthesis of -lactam antibiotics, such as cefazolin. Escherichia coli strain VKPM B-10182 produces a peptidohydrolase specific to the derivatives of phenylacetic acid, tetrazolylacetic acid, and thienylacetic acid. It is very promising for application in the industrial manufacturing of corresponding antibiotics by biocatalytic synthesis [1,2,3] and is identical to penicillin G acylase (PGA), which is involved in benzylpenicillin hydrolysis [4, 5].
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