Abstract
ABSTRACTEnterohemorrhagic Escherichia coli (EHEC) with serotype O157:H7 is a major foodborne pathogen. Here, we report the draft genome sequence of EHEC O157:H7 strain MC2 isolated from cattle in France. The assembly contains 5,400,376 bp that encoded 5,914 predicted genes (5,805 protein-encoding genes and 109 RNA genes).
Highlights
Enterohemorrhagic Escherichia coli (EHEC) with serotype O157:H7 is a major foodborne pathogen
The contigs were aligned to the E. coli Sakai genome (NCBI Reference Sequence NC_002695) and pO157 plasmid (NC_002128) using Mauve (v2.3.1)
Comparison of MC2 and Sakai genes using BLASTn (70% coverage and identity cutoff) demonstrated that 4,608 of the MC2 functional genes were present in E. coli O157:H7 Sakai, showing an average homology of 99.91%
Summary
Enterohemorrhagic Escherichia coli (EHEC) with serotype O157:H7 is a major foodborne pathogen. We sequenced E. coli MC2 because a better understanding of the nature of E. coli O157:H7 colonization of cattle is required to develop more effective preharvest food safety practices and to reduce the contamination of food of bovine origin. E. coli MC2 was cultured on Luria-Bertani medium, and the genomic DNA was extracted using the DNeasy blood and tissue kit (Qiagen). Whole-genome sequencing was performed at the GeT-PlaGe core facility (INRA Toulouse, France [http://get.genotoul.fr/]).
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